pLV-PGK-DIO-mCherry-TLR4miR-TLR2miR
(Plasmid
#137733)
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PurposeLentivirus vector for TLR2/4 miR in a Cre-dependent manner
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 137733 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLV-PGK-GFP
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Backbone manufacturerÅkerblom et al., 2013
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTKR2/4 microRNA
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gRNA/shRNA sequenceTGC TGT GAA GAG TCA GGT GAT GGA TGG TTT TGG CCA CTG ACT GAC CAT CCA TCC TGA CTC TTC A and TGC TGT TCA CGT AGA AAC TGT AAG TCG TTT TGG CCA CTG ACT GAC GAC TTA CAT TCT ACG TGA A
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SpeciesM. musculus (mouse)
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Tag
/ Fusion Protein
- mChherry (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer ccctcgttgaccgaatcacc
- 3′ sequencing primer tgacaacgggccacaactcc (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThermo Fisher Scientific
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
the website for the BLOCK-iT RNAi Designer
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-PGK-DIO-mCherry-TLR4miR-TLR2miR was a gift from Tomoyuki Furuyashiki (Addgene plasmid # 137733 ; http://n2t.net/addgene:137733 ; RRID:Addgene_137733) -
For your References section:
The Innate Immune Receptors TLR2/4 Mediate Repeated Social Defeat Stress-Induced Social Avoidance through Prefrontal Microglial Activation. Nie X, Kitaoka S, Tanaka K, Segi-Nishida E, Imoto Y, Ogawa A, Nakano F, Tomohiro A, Nakayama K, Taniguchi M, Mimori-Kiyosue Y, Kakizuka A, Narumiya S, Furuyashiki T. Neuron. 2018 Aug 8;99(3):464-479.e7. doi: 10.1016/j.neuron.2018.06.035. Epub 2018 Jul 19. 10.1016/j.neuron.2018.06.035 PubMed 30033154
Map uploaded by the depositor.
