pET15b-mLin28A-cHIS
(Plasmid
#137998)
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PurposeLin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 137998 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET15b
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemouse Lin28A, CSD and ZnFs (residues 36–186)
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SpeciesM. musculus (mouse)
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Insert Size (bp)500
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Tag
/ Fusion Protein
- C-terminal His6 tag
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15b-mLin28A-cHIS was a gift from Judy Lieberman (Addgene plasmid # 137998 ; http://n2t.net/addgene:137998 ; RRID:Addgene_137998) -
For your References section:
An RNA-binding Protein, Lin28, Recognizes and Remodels G-quartets in the MicroRNAs (miRNAs) and mRNAs It Regulates. O'Day E, Le MT, Imai S, Tan SM, Kirchner R, Arthanari H, Hofmann O, Wagner G, Lieberman J. J Biol Chem. 2015 Jul 17;290(29):17909-22. doi: 10.1074/jbc.M115.665521. Epub 2015 Jun 4. 10.1074/jbc.M115.665521 PubMed 26045559
