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pPS1304
(Plasmid #1380)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1380 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPS293
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gal-GFP
  • Insert Size (bp)
    700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer M13F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Gal-sGFP vector for C-terminal fusions. GFP ORF cloned into HindIII site of pPS293. pPS293 has the GAL1 promoter (EcoRI/XbaI fragment) in YEp352 (2 micron ori). See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS1304 was a gift from Pamela Silver (Addgene plasmid # 1380)
  • For your References section:

    The Saccharomyces cerevisiae cyclin Clb2p is targeted to multiple. Hood JK, Hwang WW, Silver PA. J Cell Sci 2001 Feb;114(Pt 3):589-97. PubMed 11171327