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pCDNA3 Flag Jnk2a2
(Plasmid #13801)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Jnk2a2
  • Alt name
    MAPK9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • GenBank ID
    NM_002752
  • Entrez Gene
    MAPK9 (a.k.a. JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 Flag Jnk2a2 was a gift from Roger Davis (Addgene plasmid # 13801)
  • For your References section:

    Signal transduction by tumor necrosis factor mediated by JNK protein kinases. Sluss HK, Barrett T, Derijard B, Davis RJ. Mol Cell Biol. 1994 Dec . 14(12):8376-84. PubMed 7969172