SIRT3 Flag
(Plasmid #13814)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3.1 +
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    SIRT3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1199
  • Entrez Gene
    SIRT3 (a.k.a. SIR2L3)
  • Tag / Fusion Protein
    • flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Roy Frye
  • Terms and Licenses

Depositor Comments

cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SIRT3 Flag was a gift from Eric Verdin (Addgene plasmid # 13814)
  • For your References section:

    The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. North BJ, Marshall BL, Borra MT, Denu JM, Verdin E. Mol Cell. 2003 Feb . 11(2):437-44. 10.1016/S1097-2765(03)00038-8 PubMed 12620231