pBEL1200
(Plasmid #138526)

• Purpose: For expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.
• Depositing Labs: Gira Bhabha, Damian Ekiert
• Publication: Ekiert et al Cell. 2017 Apr 6;169(2):273-285.e17. doi: 10.1016/j.cell.2017.03.019.

Backbone

• Vector backbone: pBAD-His
• Backbone manufacturer: Thermo Fisher
• Backbone size w/o insert (bp): 4571
• Total vector size (bp): 7728
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mlaFEDCB
• Species: E. coli
• Insert Size (bp): 3157
• Entrez Gene: mlaF (a.k.a. b3195, ECK3184, vpsA(S.f.), yrbF)
• Entrez Gene: mlaE (a.k.a. b3194, ECK3183, vpsB(S.f.), yrbE)
• Entrez Gene: mlaD (a.k.a. b3193, ECK3182, vpsC(S.f.), yrbD)
• Entrez Gene: mlaC (a.k.a. b3192, ECK3181, yrbC)
• Entrez Gene: mlaB (a.k.a. b3191, ECK3180, yrbB)
• Promoter: araBAD
• Tag / Fusion Protein:
  • N-terminal His tag and TEV cleavage site on MlaD (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pBAD fwd
• 3′ sequencing primer: pBAD rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry