PurposeFirefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17835541_17837355
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||138573||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4411
- Total vector size (bp) 6246
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCd36 enhancer
SpeciesM. musculus (mouse)
Insert Size (bp)1835
Entrez GeneCd36 (a.k.a. FAT, GPIV, Scarb3)
- Promoter Minimal promoter
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer TGGCTTTACCAACAGTACCGGA (Common Sequencing Primers)
Addgene NGS found a 5bp t deletion in the Cd36-enhancer1 region. Depositor confirms this deletion does not affect plasmid function and they observed strong induction using the luciferase assay.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL4.24-Cd36-enhancer1 was a gift from Lora Hooper (Addgene plasmid # 138573 ; http://n2t.net/addgene:138573 ; RRID:Addgene_138573)
For your References section:The intestinal microbiota programs diurnal rhythms in host metabolism through histone deacetylase 3. Kuang Z, Wang Y, Li Y, Ye C, Ruhn KA, Behrendt CL, Olson EN, Hooper LV. Science. 2019 Sep 27;365(6460):1428-1434. doi: 10.1126/science.aaw3134. 10.1126/science.aaw3134 PubMed 31604271