pGEM cBspII (CT#624)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13940||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepGEM-T Easy
- Backbone size w/o insert (bp) 3015
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namebone sialoprotein II
SpeciesG. gallus (chicken)
Insert Size (bp)530
MutationAmino acids 79-257
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
530 bp RT-PCR product corresponding to amino acids 79-257 of protein sequence published by Yang et al., 1995.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEM cBspII (CT#624) was a gift from Cliff Tabin (Addgene plasmid # 13940 ; http://n2t.net/addgene:13940 ; RRID:Addgene_13940)
For your References section:Dual roles of Wnt signaling during chondrogenesis in the chicken limb. Hartmann C, Tabin CJ. Development. 2000 Jul . 127(14):3141-59. PubMed 10862751