pGEM cOp (CT#625)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13941||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGEM-T Easy
- Backbone size w/o insert (bp) 3015
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesG. gallus (chicken)
Insert Size (bp)645
MutationAmino acids 34-260, V98M, G216D, R235A*
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
645 bp RT-PCR product corresponding to AA 34-260 of sequenced published by Castagnola et al., 1991.
*Note that these mutations were not reported but were found during Addgene's quality control sequencing. They are not known to affect function and may only be polymorphisms.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEM cOp (CT#625) was a gift from Cliff Tabin (Addgene plasmid # 13941 ; http://n2t.net/addgene:13941 ; RRID:Addgene_13941)
For your References section:Dual roles of Wnt signaling during chondrogenesis in the chicken limb. Hartmann C, Tabin CJ. Development. 2000 Jul . 127(14):3141-59. PubMed 10862751