Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pGEM cOp (CT#625)
(Plasmid #13941)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13941 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM-T Easy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3015
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Op
  • Alt name
    Osteopontin
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    645
  • Mutation
    Amino acids 34-260, V98M, G216D, R235A*
  • Entrez Gene
    SPP1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

645 bp RT-PCR product corresponding to AA 34-260 of sequenced published by Castagnola et al., 1991.

*Note that these mutations were not reported but were found during Addgene's quality control sequencing. They are not known to affect function and may only be polymorphisms.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM cOp (CT#625) was a gift from Cliff Tabin (Addgene plasmid # 13941 ; http://n2t.net/addgene:13941 ; RRID:Addgene_13941)
  • For your References section:

    Dual roles of Wnt signaling during chondrogenesis in the chicken limb. Hartmann C, Tabin CJ. Development. 2000 Jul . 127(14):3141-59. PubMed 10862751