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RCAS(A) cTbx5 VP16 (CT#630)
(Plasmid #13969)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 13969 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    RCASBP(A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tbx5
  • Species
    G. gallus (chicken)
  • Mutation
    Amino acids 1-274
  • Entrez Gene
    TBX5

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Contains amino acids 1-274 of Tbx5 fused to a duplex of the lambda hinge region and VP16 (transcriptional activator).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS(A) cTbx5 VP16 (CT#630) was a gift from Cliff Tabin (Addgene plasmid # 13969 ; http://n2t.net/addgene:13969 ; RRID:Addgene_13969)
  • For your References section:

    Tbx5 is required for forelimb bud formation and continued outgrowth. Rallis C, Bruneau BG, Del Buono J, Seidman CE, Seidman JG, Nissim S, Tabin CJ, Logan MP. Development. 2003 Jun . 130(12):2741-51. 10.1242/dev.00473 PubMed 12736217