RCAS(A) cTbx5 VP16 (CT#630)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13969||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 11600
Vector typeRetroviral ; avian expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesG. gallus (chicken)
MutationAmino acids 1-274
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Contains amino acids 1-274 of Tbx5 fused to a duplex of the lambda hinge region and VP16 (transcriptional activator).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RCAS(A) cTbx5 VP16 (CT#630) was a gift from Cliff Tabin (Addgene plasmid # 13969 ; http://n2t.net/addgene:13969 ; RRID:Addgene_13969)
For your References section:Tbx5 is required for forelimb bud formation and continued outgrowth. Rallis C, Bruneau BG, Del Buono J, Seidman CE, Seidman JG, Nissim S, Tabin CJ, Logan MP. Development. 2003 Jun . 130(12):2741-51. 10.1242/dev.00473 PubMed 12736217