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RCAS (A) Hoxd13 (CT#441)
(Plasmid #13985)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 13985 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    RCASBP(A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Hoxd-13
  • Alt name
    Hox D13
  • Alt name
    homeobox D13
  • Species
    G. gallus (chicken)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

RCASHoxd-13 was constructed using a cDNA corresponding to the longer of the two transcripts whose putative coding region is identical to the published sequence, with the exception of 9 glycines encoded by ggc repeats starting at amino acid position 10, instead of the reported 7 ggc repeats (Rogina and Upholdt, 1993). This cDNA was cloned into the shuttle vector SLAX-13 (Riddle et al., 1993), and then subcloned into the ClaI site of RCAS(BP)A .

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS (A) Hoxd13 (CT#441) was a gift from Cliff Tabin (Addgene plasmid # 13985 ; http://n2t.net/addgene:13985 ; RRID:Addgene_13985)
  • For your References section:

    Analysis of Hoxd-13 and Hoxd-11 misexpression in chick limb buds reveals that Hox genes affect both bone condensation and growth. Goff DJ, Tabin CJ. Development. 1997 Feb . 124(3):627-36. PubMed 9043077