RCAS (A) Hoxd13 (CT#441)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13985||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 11600
Vector typeRetroviral ; avian expression
Growth in Bacteria
Copy numberLow Copy
Alt nameHox D13
Alt namehomeobox D13
SpeciesG. gallus (chicken)
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
RCASHoxd-13 was constructed using a cDNA corresponding to the longer of the two transcripts whose putative coding region is identical to the published sequence, with the exception of 9 glycines encoded by ggc repeats starting at amino acid position 10, instead of the reported 7 ggc repeats (Rogina and Upholdt, 1993). This cDNA was cloned into the shuttle vector SLAX-13 (Riddle et al., 1993), and then subcloned into the ClaI site of RCAS(BP)A .
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RCAS (A) Hoxd13 (CT#441) was a gift from Cliff Tabin (Addgene plasmid # 13985 ; http://n2t.net/addgene:13985 ; RRID:Addgene_13985)
For your References section:Analysis of Hoxd-13 and Hoxd-11 misexpression in chick limb buds reveals that Hox genes affect both bone condensation and growth. Goff DJ, Tabin CJ. Development. 1997 Feb . 124(3):627-36. PubMed 9043077