Addgene - RCAS (E) shh (CT#489) Plasmid Data

Plasmid 13992: RCAS (E) shh (CT#489)

Gene/insert name:
shh
Alt name:
sonic hedgehog
Insert size:
1500
Species:
G. gallus (chicken)
Entrez Gene:
SHH ()
Vector backbone:
RCASBP(E)
(Search Vector Database)
Vector type:
Retroviral ; avian expression
Backbone size w/o insert (bp):
11600
Cloning site 5':
ClaI
Site destroyed during cloning:
No
Cloning site 3':
ClaI
Site destroyed during cloning:
No
5' sequencing primer:
RCAS-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Cliff Tabin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Sonic hedgehog mediates the polarizing activity of the ZPA. Riddle et al (Cell. 1993 Dec 31. 75(7):1401-16. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13992" in your Materials and Methods section.