pCMV-T7-SpCas9-P2A-EGFP (RTW3027) Citations (4)
Originally described in: Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.Walton RT, Christie KA, Whittaker MN, Kleinstiver BP Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26. PubMed Journal
Articles Citing pCMV-T7-SpCas9-P2A-EGFP (RTW3027)
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Precise DNA cleavage using CRISPR-SpRYgests.
Christie KA, Guo JA, Silverstein RA, Doll RM, Mabuchi M, Stutzman HE, Lin J, Ma L, Walton RT, Pinello L, Robb GB, Kleinstiver BP.
Nat Biotechnol. 2022 Oct 6. pii: 10.1038/s41587-022-01492-y. doi: 10.1038/s41587-022-01492-y.
PubMed
Associated Plasmids |
| In situ architecture of Opa1-dependent mitochondrial cristae remodeling. Fry MY, Navarro PP, Qin X, Inde Z, Ananda VY, Lugo CM, Hakim P, Luce BE, Ge Y, McDonald JL, Ali I, Ha LL, Kleinstiver BP, Chan DC, Sarosiek KA, Chao LH. bioRxiv. 2023 Feb 15:2023.01.16.524176. doi: 10.1101/2023.01.16.524176. Preprint. PubMed |
| PMS1 as a target for splice modulation to prevent somatic CAG repeat expansion in Huntington's disease. McLean ZL, Gao D, Correia K, Roy JCL, Shibata S, Farnum IN, Valdepenas-Mellor Z, Rapuru M, Morini E, Ruliera J, Gillis T, Lucente D, Kleinstiver BP, Lee JM, MacDonald ME, Wheeler VC, Pinto RM, Gusella JF. bioRxiv [Preprint]. 2023 Jul 27:2023.07.25.550489. doi: 10.1101/2023.07.25.550489. PubMed |
PAM-flexible genome editing with an engineered chimeric Cas9.
Zhao L, Koseki SRT, Silverstein RA, Amrani N, Peng C, Kramme C, Savic N, Pacesa M, Rodriguez TC, Stan T, Tysinger E, Hong L, Yudistyra V, Ponnapati MR, Jacobson JM, Church GM, Jakimo N, Truant R, Jinek M, Kleinstiver BP, Sontheimer EJ, Chatterjee P.
Nat Commun. 2023 Oct 4;14(1):6175. doi: 10.1038/s41467-023-41829-y.
PubMed
Associated Plasmids |
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