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pAKlux2
(Plasmid #14080)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14080 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Bacterial luciferase
  • Alt name
    luxCDABE
  • Insert Size (bp)
    5843

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAKlux2 was a gift from Attila Karsi (Addgene plasmid # 14080 ; http://n2t.net/addgene:14080 ; RRID:Addgene_14080)
  • For your References section:

    Broad host range fluorescence and bioluminescence expression vectors for Gram-negative bacteria. Karsi A, Lawrence ML. Plasmid. 2007 Jan 4. ():. 10.1016/j.plasmid.2006.11.002 PubMed 17207855