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(Plasmid #1410)


Item Catalog # Description Quantity Price (USD)
Plasmid 1410 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha)
  • Alt name
    p85 alpha
  • Alt name
    p110 alpha
  • Alt name
    Pik3r1, Pik3ca
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Pik3ca (a.k.a. 6330412C24Rik, caPI3K, p110, p110alpha)
  • Entrez Gene
    Pik3r1 (a.k.a. PI3K, p50alpha, p55alpha, p85alpha)
  • Promoter SRalpha promoter
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer SV40pro-F2 (5'-CCCCATGGCTGACTAATTTTT-3')
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please note that NcoI is not a unique enzyme and will cut the plasmid DNA at five locations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myr-(iSH2-p85)-p110alpha-Myc was a gift from Lewis Cantley (Addgene plasmid # 1410 ; ; RRID:Addgene_1410)