pMW-PahZ1 KT-1
(Plasmid #141134)

• Purpose: Expresses in BL-21 (DE3) cells to produce the poly(aspartic acid) hydrolase-1 from Sphingomonas Sp. KT-1 which selectively cleaves the beta linkages in thermally synthesized poly(aspartic acid).
• Depositing Lab: Mitch Weiland
• Publication: Bolay et al Journal of Chemical Education

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5709
• Total vector size (bp): 6533
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PahZ1
• Alt name: Poly(aspartic acid) hydrolase-1 KT-1
• Alt name: PAAH-1
• Species: Sphingomonas Sp. KT-1
• Insert Size (bp): 825
• Mutation: Not the full-length gene. PahZ1 fragment starts at residue 41
• GenBank ID: BAC78155.1
• Tag / Fusion Protein:
  • 6x His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7 Promoter Primer
• 3′ sequencing primer: T7 Terminator Primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Genscript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see doi 10.1021/acssuschemeng.0c01158 for more detail about how this plasmid was created.