pDONR207 SARS-CoV-2 NSP3
PurposeGateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||141257||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
|Cloning Grade DNA||141257-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95 *|
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- Backbone size w/o insert (bp) 5585
Vector typeGateway-compatible Entry vector
Growth in Bacteria
Alt nameGene ID 43740578
SpeciesSARS-CoV-2 isolate Wuhan-Hu-1
Insert Size (bp)5841
MutationMany synonymous changes due to codon optimization
Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Cloning method Gateway Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Information for Cloning Grade DNA (Catalog # 141257-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDONR207 SARS-CoV-2 NSP3 was a gift from Fritz Roth (Addgene plasmid # 141257 ; http://n2t.net/addgene:141257 ; RRID:Addgene_141257)
For your References section:A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP. G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. 10.1534/g3.120.401554 PubMed 32763951