pcDNA3 Flag TSC2 (S939A/T1462A)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14131||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerFLAG (MDYKDDDDK) was cloned between HindIII and BamHI of pcDNA3
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameTSC2 S939A/T1462A
SpeciesH. sapiens (human)
Insert Size (bp)5400
Entrez GeneTSC2 (a.k.a. LAM, PPP1R160, TSC4)
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The first 276 base pairs of TSC2 were PCR-amplified from pcDNA6-TSC2 and inserted between the BamHI and NotI sites in frame with the FLAG-coding sequence in pcDNA3-FLAG. The remainder of TSC2 was subcloned from pcDNA6-TSC2 and inserted between the NotI and XbaI sites of the above construct. The pcDNA3-FLAG-tuberinS939A/T1462A construct was made by inserting the Bsu36I to EcoRV fragment of pcDNA3-FLAG-tuberinT1462A into these same sites on pcDNA3-FLAG-tuberinS939A.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 Flag TSC2 (S939A/T1462A) was a gift from Brendan Manning (Addgene plasmid # 14131 ; http://n2t.net/addgene:14131 ; RRID:Addgene_14131)
For your References section:Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/akt pathway. Manning BD, Tee AR, Logsdon MN, Blenis J, Cantley LC. Mol Cell. 2002 Jul . 10(1):151-62. 10.1016/S1097-2765(02)00568-3 PubMed 12150915