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pcDNA3 Flag TSC2 (S939A/T1462A)
(Plasmid #14131)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14131 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3-Flag
  • Backbone manufacturer
    FLAG (MDYKDDDDK) was cloned between HindIII and BamHI of pcDNA3
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TSC2 S939A/T1462A
  • Alt name
    tuberin
  • Alt name
    TSC2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5400
  • Mutation
    S939A/T1462A
  • Entrez Gene
    TSC2 (a.k.a. LAM, PPP1R160, TSC4)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The first 276 base pairs of TSC2 were PCR-amplified from pcDNA6-TSC2 and inserted between the BamHI and NotI sites in frame with the FLAG-coding sequence in pcDNA3-FLAG. The remainder of TSC2 was subcloned from pcDNA6-TSC2 and inserted between the NotI and XbaI sites of the above construct. The pcDNA3-FLAG-tuberinS939A/T1462A construct was made by inserting the Bsu36I to EcoRV fragment of pcDNA3-FLAG-tuberinT1462A into these same sites on pcDNA3-FLAG-tuberinS939A.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 Flag TSC2 (S939A/T1462A) was a gift from Brendan Manning (Addgene plasmid # 14131 ; http://n2t.net/addgene:14131 ; RRID:Addgene_14131)
  • For your References section:

    Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/akt pathway. Manning BD, Tee AR, Logsdon MN, Blenis J, Cantley LC. Mol Cell. 2002 Jul . 10(1):151-62. 10.1016/S1097-2765(02)00568-3 PubMed 12150915