Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro
(Plasmid #141368)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 141368 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

* Login to view industry pricing.

Backbone

  • Vector backbone
    pLVX-EF1alpha-IRES-Puro
  • Backbone manufacturer
    Takara Bio
  • Backbone size w/o insert (bp) 8825
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2-nsp2
  • Species
    SARS-CoV-2
  • Mutation
    human codon optimized
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter EF1alpha
  • Tag / Fusion Protein
    • 2xStrep (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141368 ; http://n2t.net/addgene:141368 ; RRID:Addgene_141368)
  • For your References section:

    A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug Repurposing. Gordon DE, et al.. BioRxiv March 2020 10.1101/2020.03.22.002386