pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro
(Plasmid #141395)

• Purpose: Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
• Depositing Lab: Nevan Krogan
• Publication: Gordon et al BioRxiv March 2020

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	141395	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pLVX-EF1alpha-IRES-Puro
  (Search Vector Database)
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eGFP
• Species: jellyfish
• Mutation: human codon optimized
• Promoter: EF1alpha
• Tag / Fusion Protein:
  • 2xStrep (C terminal on insert)

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro.gb
• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry