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PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 141395 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLVX-EF1alpha-IRES-Puro
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeGFP
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Speciesjellyfish
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Mutationhuman codon optimized
- Promoter EF1alpha
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Tag
/ Fusion Protein
- 2xStrep (C terminal on insert)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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Terms and Licenses
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Industry Terms
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141395 ; http://n2t.net/addgene:141395 ; RRID:Addgene_141395) -
For your References section:
A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug Repurposing. Gordon DE, et al.. BioRxiv March 2020 10.1101/2020.03.22.002386