Addgene - pFLAG-GATA2 Plasmid Data

Plasmid 1418: pFLAG-GATA2

Gene/insert name:
GATA-2
Alt name:
GATA2
Insert size:
2200
Species:
H. sapiens (human)
GenBank ID:
NM_032638
Entrez Gene:
GATA2 (DCML, MONOMAC, NFE1B)
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Vector backbone:
pFLAG-CMV2
(Search Vector Database)
Backbone manufacturer:
Sigma Aldrich
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
EcoRV
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRV
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV promoter List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Gokhan Hotamisligil
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Interaction between GATA and the C/EBP family of transcription factors is critical in GATA-mediated suppression of adipocyte differentiation. Tong et al (Mol Cell Biol 2005 Jan;25(2):706-15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1418" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

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