Addgene - mRFP-Rab5 Plasmid Data

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14437: mRFP-Rab5
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Plasmid 14437: mRFP-Rab5

Gene/insert name:
Rab 5
Alt name:
Rab5
Insert size:
663
Species:
H. sapiens (human)
GenBank ID:
NM_004162
Entrez Gene:
RAB5A (RAB5)
Fusion protein or tag:
mRFP
Terminal:
N terminal on backbone
Vector backbone:
pmRFP-C3
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4656
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
GACTACACCATCGTGGAA List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Ari Helenius
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Rab7 associates with early endosomes to mediate sorting and transport of Semliki forest virus to late endosomes. Vonderheit et al (PLoS Biol. 2005 Jul . 3(7):e233. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14437" in your Materials and Methods section.