Addgene - p3259 pCMV hUBC9 mt HA Plasmid Data

Plasmid 14439: p3259 pCMV hUBC9 mt HA

Gene/insert name:
UBC9 C93S
Alt name:
UBC 9
Alt name:
hUBC9
Insert size:
500
Species:
H. sapiens (human)
Entrez Gene:
UBE2I (LA16c-358B7.1, C358B7.1, P18, UBC9)
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Mutation:
Cys93Ser mutation (TGC changed to AGC)
Vector backbone:
pCMV4
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4900
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
BclI/BglII
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Peter Howley
Terms and Licenses:
MTA

Comments:

A fragment containing the full coding region of hubc9 C93S cDNA along with the influenza virus hemagglutinin 1 epitope (HA) sequence (YPYDVPDYA) at the 3′-end was produced with appropriate synthetic oligonucleotides using PCR. The fragment was then cloned into the pCMV4 vector at the BglII site.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9. Yasugi et al (Nucleic Acids Res. 1996 Jun 1. 24(11):2005-10. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14439" in your Materials and Methods section.