pcDNA3.1-kappa-myc-dK-2xG4S-TMst
(Plasmid #145766)

• Purpose: Expresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.
• Depositing Lab: Marcel Bruchez
• Publication: Carpenter et al ACS Chem Biol. 2020 Aug 17. doi: 10.1021/acschembio.0c00419.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5358
• Total vector size (bp): 6459
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: kappa-myc-dK-2xG4S-TMst
• Alt name: dK-TM
• Alt name: dl5(E52K)-TM
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1101
• Mutation: The dK FAP is dL5 with mutations at E50K, L89S, also known as E52K/L91S and NP138
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMVF
• 3′ sequencing primer: BGHR

Resource Information

• Supplemental Documents:
  • pcDNA3.1-kappa-myc-dL5E52K_G25S-2XG4S-TMst.str

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.

http://pathways.mbic.cmu.edu/

For more information about this plasmid, please see the associated publication (PMID: 32786268).