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(Plasmid #14579)

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Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.


  • Gene/Insert name
    2-nitropropane dioxygenase
  • Alt name
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PA1024_pET21a(+)C-tag was a gift from SeWon Suh (Addgene plasmid # 14579)
  • For your References section:

    Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base. Ha JY, Min JY, Lee SK, Kim HS, Kim do J, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW. J Biol Chem. 2006 Jul 7. 281(27):18660-7. 10.1074/jbc.M601658200 PubMed 16682407