Addgene - PA1024_pET21a(+)C-tag Plasmid Data

Plasmid 14579: PA1024_pET21a(+)C-tag

Gene/insert name:
2-nitropropane dioxygenase
Alt name:
PA1024
Insert size:
987
Species:
Pseudomonas aeruginosa
GenBank ID:
NP_249715
Entrez Gene:
PA1024 ()
Fusion protein or tag:
His
Terminal:
C terminal on insert
Vector backbone:
pET-21a(+)
(Search Vector Database)
Backbone manufacturer:
Merck
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5443
Cloning site 5':
NdeI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (1)
Principal Investigator:
SeWon Suh
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base. Ha et al (J Biol Chem. 2006 Jul 7. 281(27):18660-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14579" in your Materials and Methods section.

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