Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

PA1024_pET21a(+)C-tag
(Plasmid #14579)

Loading...
Add to Cart
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pET-21a(+)
  • Backbone manufacturer
    Merck
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    2-nitropropane dioxygenase
  • Alt name
    PA1024
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    987
  • GenBank ID
    NP_249715
  • Entrez Gene
    PA1024
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PA1024_pET21a(+)C-tag was a gift from SeWon Suh (Addgene plasmid # 14579)
  • For your References section:

    Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base. Ha JY, Min JY, Lee SK, Kim HS, Kim do J, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW. J Biol Chem. 2006 Jul 7. 281(27):18660-7. 10.1074/jbc.M601658200 PubMed 16682407