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Plasmid 14579: PA1024_pET21a(+)C-tag
  • 2-nitropropane dioxygenase

  • PA1024

  • 987

  • Pseudomonas aeruginosa

  • NP_249715

  • PA1024 ()

  • His

  • C terminal on insert

  • pET-21a(+)
    (Search Vector Database)

  • Merck

  • Bacterial Expression

  • 5443

  • NdeI

  • No

  • XhoI

  • No

  • T7 List of Sequencing Primers

  • T7

  • Ampicillin

  • DH5alpha

  • 37

  • Low Copy

  • View sequences (1)
  • SeWon Suh

  • UBMTA
    EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base. Ha et al (J Biol Chem. 2006 Jul 7. 281(27):18660-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14579" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with