pGal CycK (P#844)
(Plasmid #14664)

• Depositing Lab: Matija Peterlin
• Publication: Lin et al J Biol Chem. 2002 May 10. 277(19):16873-8.

Backbone

• Vector backbone: pSG424
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CycK
• Alt name: cyclin K
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Entrez Gene: CCNK (a.k.a. CPR4, IDDHDF)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid pGal (pSG424) contains the coding sequence of GAL4-(1-147) immediately followed by a multiple cloning site.