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EMSV-myogenin (puro) Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid #14712 with the enzyme HindIII-HF.

We expected the following banding pattern:

HindIII-HF single digest: 4.3KB, 2.2KB and 0.5KB

The 0.5KB band appears to be missing from this plasmid. Instead there is a faint band at ~1KB. It is unclear whether this discrepancy affects plasmid function.