|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14746||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Insert Size (bp)1200
Entrez GeneMAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MKK1, PRKMK1)
/ Fusion Protein
- EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
This plasmid grows slowly. We encourage requesting scientists to allow the plasmid to grow for a longer period of time than normal.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MEK1-GFP was a gift from Rony Seger (Addgene plasmid # 14746 ; http://n2t.net/addgene:14746 ; RRID:Addgene_14746)
For your References section:Non-regulated and stimulated mechanisms cooperate in the nuclear accumulation of MEK1. Yao Z, Flash I, Raviv Z, Yung Y, Asscher Y, Pleban S, Seger R. Oncogene. 2001 Nov 15. 20(52):7588-96. 10.1038/sj.onc.1204963 PubMed 11753637
Map uploaded by the depositor.