MEK1-GFP
(Plasmid #14746)

• Depositing Lab: Rony Seger
• Publication: Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MEK1
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Entrez Gene: MAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid grows slowly. We encourage requesting scientists to allow the plasmid to grow for a longer period of time than normal.