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MSCV let-7e mutant
(Plasmid #14783)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14783 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    MSCV-neo
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    let-7e mutant
  • Alt name
    let-7e
  • Species
    M. musculus (mouse)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

miRNA of the following mature sequence, with approximately 500 basepairs of flanking sequence, were cloned into MSCV-neo: seed mutant let-7e: 5’-UCUGG ACGGA GGUUG UAUAG U-3’.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV let-7e mutant was a gift from Tyler Jacks (Addgene plasmid # 14783)

  • For your References section:

    Impaired microRNA processing enhances cellular transformation and tumorigenesis. Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T. Nat Genet. 2007 May;39(5):673-7. doi: 10.1038/ng2003. 10.1038/ng2003 PubMed 17401365
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