p53 KO neo
(Plasmid #14825)

• Depositing Lab: Tyler Jacks
• Publication: Jacks et al Curr Biol. 1994 Jan 1. 4(1):1-7.

Backbone

• Vector backbone: pPNT
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p53KO
• Alt name: p53 neo
• Alt name: p53
• Species: M. musculus (mouse)
• Insert Size (bp): 9500
• Mutation: The neomycin gene is flanked by portions of the p53 gene
• Entrez Gene: Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

p53KO was constructed by cloning fragments of the murine p53 gene (isolated from strain BALB/c) into the targeting vector pPNT. The neo gene in p53KO is flanked by a 3.5 kb fragment extending from the Bam HI site in intron 1 to the NcoI in exon 2 and a 5 kb fragment extending from the Bam HI in intron 6 to the Eco RI downstream of exon 11.