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p53 KO neo
(Plasmid #14825)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pPNT
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    p53KO
  • Alt name
    p53 neo
  • Alt name
    p53
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    9500
  • Mutation
    The neomycin gene is flanked by portions of the p53 gene
  • Entrez Gene
    Trp53 (a.k.a. RP23-56I20.1, Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

p53KO was constructed by cloning fragments of the murine p53 gene (isolated from strain BALB/c) into the targeting vector pPNT. The neo gene in p53KO is flanked by a 3.5 kb fragment extending from the Bam HI site in intron 1 to the NcoI in exon 2 and a 5 kb fragment extending from the Bam HI in intron 6 to the Eco RI downstream of exon 11.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p53 KO neo was a gift from Tyler Jacks (Addgene plasmid # 14825)
  • For your References section:

    Tumor spectrum analysis in p53-mutant mice. Jacks T, Remington L, Williams BO, Schmitt EM, Halachmi S, Bronson RT, Weinberg RA. Curr Biol. 1994 Jan 1. 4(1):1-7. 10.1016/S0960-9822(00)00002-6 PubMed 7922305