Addgene - p53 KO neo Plasmid Data

Plasmid 14825: p53 KO neo

Gene/insert name:
p53KO
Alt name:
p53 neo
Alt name:
p53
Insert size:
9500
Species:
M. musculus (mouse)
Entrez Gene:
Trp53 (RP23-56I20.1, Tp53, bbl, bfy, bhy, p44, p53)
Mutation:
The neomycin gene is flanked by portions of the p53 gene
Vector backbone:
pPNT
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6000
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

p53KO was constructed by cloning fragments of the murine p53 gene (isolated from strain BALB/c) into the targeting vector pPNT. The neo gene in p53KO is flanked by a 3.5 kb fragment extending from the Bam HI site in intron 1 to the NcoI in exon 2 and a 5 kb fragment extending from the Bam HI in intron 6 to the Eco RI downstream of exon 11.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Tumor spectrum analysis in p53-mutant mice. Jacks et al (Curr Biol. 1994 Jan 1. 4(1):1-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14825" in your Materials and Methods section.