p53 KO neo
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14825||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Alt namep53 neo
SpeciesM. musculus (mouse)
Insert Size (bp)9500
MutationThe neomycin gene is flanked by portions of the p53 gene
Entrez GeneTrp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
p53KO was constructed by cloning fragments of the murine p53 gene (isolated from strain BALB/c) into the targeting vector pPNT. The neo gene in p53KO is flanked by a 3.5 kb fragment extending from the Bam HI site in intron 1 to the NcoI in exon 2 and a 5 kb fragment extending from the Bam HI in intron 6 to the Eco RI downstream of exon 11.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p53 KO neo was a gift from Tyler Jacks (Addgene plasmid # 14825)
For your References section:Tumor spectrum analysis in p53-mutant mice. Jacks T, Remington L, Williams BO, Schmitt EM, Halachmi S, Bronson RT, Weinberg RA. Curr Biol. 1994 Jan 1. 4(1):1-7. 10.1016/S0960-9822(00)00002-6 PubMed 7922305
Generated by Addgene from full sequence.
Map uploaded by the depositor.