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Plasmid 14825: p53 KO neo
  • p53KO

  • p53 neo

  • p53

  • 9500

  • M. musculus (mouse)

  • Trp53 (RP23-56I20.1, Tp53, bbl, bfy, bhy, p44, p53)

  • The neomycin gene is flanked by portions of the p53 gene

  • pPNT
    (Search Vector Database)

  • Mammalian Expression

  • 6000

  • n/a List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • Neomycin

  • View sequences (2)
  • View map

  • Tyler Jacks

  • UBMTA

Comments: 

p53KO was constructed by cloning fragments of the murine p53 gene (isolated from strain BALB/c) into the targeting vector pPNT. The neo gene in p53KO is flanked by a 3.5 kb fragment extending from the Bam HI site in intron 1 to the NcoI in exon 2 and a 5 kb fragment extending from the Bam HI in intron 6 to the Eco RI downstream of exon 11.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Tumor spectrum analysis in p53-mutant mice. Jacks et al (Curr Biol. 1994 Jan 1. 4(1):1-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14825" in your Materials and Methods section.