pBabe Flag Smad7
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14836||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBabe puro 3
- Backbone size w/o insert (bp) 5100
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1300
Entrez GeneSmad7 (a.k.a. Madh, Madh7)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer pBabe 5' (Common Sequencing Primers)
See Author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe Flag Smad7 was a gift from Rik Derynck (Addgene plasmid # 14836 ; http://n2t.net/addgene:14836 ; RRID:Addgene_14836)
For your References section:Roles of autocrine TGF-beta receptor and Smad signaling in adipocyte differentiation. Choy L, Skillington J, Derynck R. J Cell Biol. 2000 May 1. 149(3):667-82. 10.1083/jcb.149.3.667 PubMed 10791980
Map uploaded by the depositor.