GolgiCFP
(Plasmid #14873)

• Depositing Lab: Alexandra Newton
• Publication: Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-56.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CFP
• Tag / Fusion Protein:
  • eNOS(1-35) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Sequences encoding the amino-terminal 35 residues of endothelial nitric-oxide synthase were fused in frame to the 5'-end of CFP.