HLA-A2.1
(Plasmid #14904)

• Depositing Lab: Linda Sherman
• Publication: Irwin et al J Exp Med. 1989 Oct 1. 170(4):1091-101.

Backbone

• Vector backbone: pUC9
• Backbone size w/o insert (bp): 2700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HLA-A2.1
• Alt name: HLA-A
• Species: H. sapiens (human)
• Insert Size (bp): 5100
• Entrez Gene: HLA-A (a.k.a. HLAA)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13R
• 3′ sequencing primer: M13-F20

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Harry Orr

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, see Koller BH and Orr HT, J Immunol. 1985 Apr; 134(4):2727-33.