pTCO2
(Plasmid #149428)

• Purpose: (Empty Backbone) cloning vector with regulatory regions of the murine Tdg gene
• Depositing Lab: Primo Schaer
• Publication: Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.12688/f1000research.25637.2. eCollection 2020.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Backbone size (bp): 3000
• Modifications to backbone: multiple cloning steps to generate a mammalian expression vector containing (1) about 6 and 3 kb of the promoter and the 3'UTR/terminator sequences of murine TDG (https://www.ncbi.nlm.nih.gov/gene/21665), respectively, (2) puromycin selection marker controlled by the SV40 promoter/poly(A) signal, and (3) two loxP sites in the Tdg promoter and 3' of the MCS
• Vector type: Mammalian Expression, Cre/Lox
• Promoter: murine Tdg promoter
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13/pUC forward
• 3′ sequencing primer: M13/pUC reverse

Resource Information

• Supplemental Documents:
  • pTCO2.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry