pBigTB-FlpERT2
(Plasmid #149435)

• Purpose: tamoxifen-inducible Flp recombinase
• Depositing Lab: Primo Schaer
• Publication: Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.12688/f1000research.25637.2. eCollection 2020.

Backbone

• Vector backbone: pBigTB (Addgene Plasmid #149432)
• Backbone manufacturer: Primo Schär Lab
• Backbone size w/o insert (bp): 4573
• Total vector size (bp): 8517
• Vector type: Mammalian Expression, Mouse Targeting, Cre/Lox
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Flp-ERT2 fusion protein
• Alt name: inducible Flp recombinase
• Species: Synthetic
• Insert Size (bp): 3968
• Promoter: CAG promoter
• Tag / Fusion Protein:
  • ERT2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pBigTB-FlpERT2.gbk
• A portion of this plasmid was derived from a plasmid made by: Connie Cepko Lab: pCAG-FlpeERT2 (Addgene Plasmid #14756)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry