Addgene - CS2 Flag-Smad3 (EPSM) Plasmid Data

Plasmid 14963: CS2 Flag-Smad3 (EPSM)

Gene/insert name:
Smad3 (EPSM)
Alt name:
Smad3
Insert size:
1300
Species:
H. sapiens (human)
Entrez Gene:
SMAD3 (HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Mutation:
T178V, S203A, S207A, S212A
Vector backbone:
pCS2
(Search Vector Database)
Vector type:
xenopus/mammalian/avian/zebrafish
Backbone size w/o insert (bp):
4100
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
EBV rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Joan Massague
Terms and Licenses:
MTA

Comments:

3SP and 1TP sites in the linker are mutated to alanine or valine (T-DV). See author's map for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A mechanism of repression of TGFbeta/ Smad signaling by oncogenic Ras. Kretzschmar et al (Genes Dev. 1999 Apr 1. 13(7):804-16. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14963" in your Materials and Methods section.