pBS XFS-319 (DM#133)
(Plasmid
#15057)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15057 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBlueScript SK II
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFollistatin
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Alt nameXFS
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SpeciesX. laevis (frog)
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Insert Size (bp)1173
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Entrez Genefst (a.k.a. FS, xfs)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Not I (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Short form of Xenopus follistatin. Contains ORF with ~100 bp of 5' and 3' UTR. See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS XFS-319 (DM#133) was a gift from Douglas Melton (Addgene plasmid # 15057) -
For your References section:
Follistatin, an antagonist of activin, is expressed in the Spemann organizer and displays direct neuralizing activity. Hemmati-Brivanlou A, Kelly OG, Melton DA. Cell. 1994 Apr 22. 77(2):283-95. 10.1016/0092-8674(94)90320-4 PubMed 8168135
Map uploaded by the depositor.