pCAGGS mutated Pdx1 (DM#233)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15072||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesG. gallus (chicken)
MutationDNA binding mutant
- Cloning method Restriction Enzyme
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
Mutated Pdx-1 protein is unable to bind DNA; made by changing the leucine between helices 2 and 3 of the homeodomain into a proline.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAGGS mutated Pdx1 (DM#233) was a gift from Douglas Melton (Addgene plasmid # 15072 ; http://n2t.net/addgene:15072 ; RRID:Addgene_15072)
For your References section:Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes. Grapin-Botton A, Majithia AR, Melton DA. Genes Dev. 2001 Feb 15. 15(4):444-54. 10.1101/gad.846001 PubMed 11230152