HPV-31neo
(Plasmid #153283)

• Purpose: Neomycin selectable HPV31 genome
• Depositing Lab: Elliot Androphy
• Publication: HPV-31 tyrosine 102 regulates interaction with E2 binding partners and episomal maintenance (unpublished)

Backbone

• Vector backbone: pBR322min
• Backbone size w/o insert (bp): 2600
• Total vector size (bp): 10459
• Vector type: Unspecified
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HPV-31 genome with Neo cassette
• Species: HPV-31
• Mutation: HindIII and CsiI site mutated from original Neo cassette to aid in subcloning
• GenBank ID: J04353.1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: ccacctgacgtctaag

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: this plasmid contains a 10bp deletion in the HPV origin, which is a minor variant of the genome which does not interfere with replication or gene expression. The nucleotide numbers included in the plasmid diagram do not exactly match the Addgene verified sequence. Please use this diagram as a general reference only and refer to GenBank ID J04353.1 to locate gene positions.