Addgene - FLAG ELL 500-621 pBacPAK8 Plasmid Data

Plasmid 15331: FLAG ELL 500-621 pBacPAK8

Gene/insert name:
eleven nineteen lysine rich in leukemia
Alt name:
ELL
Insert size:
366
Species:
H. sapiens (human)
Entrez Gene:
ELL (C19orf17, DKFZp434I1916, ELL1, MEN)
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Mutation:
Amino acids 500-621 only
Vector backbone:
pBacPAK8
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Baculovirus construction
Backbone size w/o insert (bp):
5538
Cloning site 5':
Sal1
Site destroyed during cloning:
Yes
Cloning site 3':
Kpn1
Site destroyed during cloning:
No
5' sequencing primer:
Bac1 List of Sequencing Primers
3' sequencing primer:
Bac2
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Joan Conaway
Principal Investigator:
Ronald Conaway
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL. Kong et al (Proc Natl Acad Sci U S A. 2005 Jul 19. 102(29):10094-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15331" in your Materials and Methods section.