pSETT4tip
(Plasmid #153414)

• Purpose: (Empty Backbone) φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette
• Depositing Lab: Joern Kalinowski
• Publication: pSETT4, an improved φC31-based integrative vector system for Actinoplanes sp. SE50/110 (unpublished)

Backbone

• Vector backbone: pSET152
• Backbone size (bp): 5715
• Modifications to backbone: The pSETT4 vector is integrating into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4-terminators are surrounding the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in further Actinomycetales by exchanging the promoter.
• Vector type: Bacterial Expression
• Promoter: tipA promoter from Streptomyces lividans
• Selectable markers: Apramycin

Growth in Bacteria

• Bacterial Resistance(s): Apramycin, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pSETT4tip.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry