Donor-CD55-NeoR
(Plasmid #153551)

• Purpose: Used as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassette
• Depositing Lab: Hiroyuki Konishi
• Publication: Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7. doi: 10.1016/j.celrep.2019.12.064.

Backbone

• Vector backbone: pAAV-2Aneo (Addgene #80032)
• Backbone manufacturer: Konishi lab
• Backbone size w/o insert (bp): 4507
• Total vector size (bp): 5903
• Modifications to backbone: Frame Adjuster in pAAV-2Aneo was truncated by digestion with BsrGI followed by self-ligation
• Vector type: AAV, Cre/Lox ; Donor plasmid/Targeting vector
• Selectable markers: Neomycin (select with G418) ; NeoR/KanR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
• Alt name: N/A
• Species: H. sapiens (human)
• Insert Size (bp): 1415
• Mutation: a 1-bp mutation in exon 4
• Promoter: No promoter
• Tag / Fusion Protein:
  • N/A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry