FLAG-Med9 pBacPAK8
(Plasmid
#15363)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15363 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBacPAK8
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5538
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Vector typeBaculovirus construction
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMed9
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Alt nameMed25
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Alt nameFLJ10193
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SpeciesM. musculus (mouse)
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Insert Size (bp)430
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Entrez GeneMed9 (a.k.a. Med25, BC019367, FLJ10193, MGC30366)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1/Nhe1 (destroyed during cloning)
- 3′ cloning site Kpn1 (not destroyed)
- 5′ sequencing primer Bac1
- 3′ sequencing primer Bac2 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-Med9 pBacPAK8 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15363 ; http://n2t.net/addgene:15363 ; RRID:Addgene_15363) -
For your References section:
A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW. J Biol Chem. 2004 Feb 13. 279(7):5846-51. 10.1074/jbc.M312523200 PubMed 14638676