Addgene - F-MED26 Plasmid Data

Plasmid 15367: F-MED26

Gene/insert name:
MED26
Alt name:
CRSP70
Alt name:
CRSP7
Insert size:
1803
Species:
H. sapiens (human)
GenBank ID:
NM_004831
Entrez Gene:
MED26 (CRSP7, CRSP70)
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Mutation:
N/A
Vector backbone:
pcDNA3.1 N-FLAG/Hyg
(Search Vector Database)
Backbone manufacturer:
Invitrogen, modified at Conaway lab
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5600
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
Yes
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
BGH reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Joan Conaway
Principal Investigator:
Ronald Conaway
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Sato et al (Mol Cell. 2004 Jun 4. 14(5):685-91. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15367" in your Materials and Methods section.