Addgene - LB1376 (Bcl-2 P1 promoter with MHS1234) Plasmid Data

Plasmid 15387: LB1376 (Bcl-2 P1 promoter with MHS1234)

Gene/insert name:
B-cell lymphoma/leukemia-2
Alt name:
Bcl-2
Insert size:
3000
Species:
H. sapiens (human)
Entrez Gene:
BCL2 (Bcl-2, PPP1R50)
Fusion protein or tag:
Luciferase
Terminal:
C terminal on backbone
Mutation:
Bcl-2 P1 promoter with IgH full enhancer MHS1234
Vector backbone:
pREP4
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Bacterial Expression, Luciferase
Backbone size w/o insert (bp):
10183
Cloning site 5':
Afl II
Site destroyed during cloning:
No
Cloning site 3':
Afl II
Site destroyed during cloning:
No
5' sequencing primer:
pREP_fwd List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Hygromycin
Map:
View map
Principal Investigator:
Linda Boxer
Terms and Licenses:
MTA
Luciferase Limited Use Label License

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: The immunoglobulin heavy-chain gene 3' enhancers deregulate bcl-2 promoter usage in t(14;18) lymphoma cells. Duan et al (Oncogene. 2007 Apr 19. 26(18):2635-41. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15387" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with