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LB1360 (Bcl-2 promoter with MHS4)
(Plasmid #15392)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pREP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10183
  • Vector type
    Mammalian Expression, Bacterial Expression, Luciferase
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    B-cell lymphoma/leukemia-2
  • Alt name
    Bcl-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1381
  • Mutation
    Bcl-2 full promoter with IgH enhancers MHS4
  • Entrez Gene
    BCL2 (a.k.a. Bcl-2, PPP1R50)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (not destroyed)
  • 3′ cloning site See map (not destroyed)
  • 5′ sequencing primer pREP_fwd
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LB1360 (Bcl-2 promoter with MHS4) was a gift from Linda Boxer (Addgene plasmid # 15392)
  • For your References section:

    The immunoglobulin heavy-chain gene 3' enhancers deregulate bcl-2 promoter usage in t(14;18) lymphoma cells. Duan H, Heckman CA, Boxer LM. Oncogene. 2007 Apr 19. 26(18):2635-41. 10.1038/sj.onc.1210061 PubMed 17043638