|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15395||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameUpstream region of mouse Esg1 pseudogene
SpeciesM. musculus (mouse)
Insert Size (bp)6000
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGV-2U (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEsg1-ps-6K-Luc was a gift from Shinya Yamanaka (Addgene plasmid # 15395 ; http://n2t.net/addgene:15395 ; RRID:Addgene_15395)
For your References section:Identification and targeted disruption of the mouse gene encoding ESG1 (PH34/ECAT2/DPPA5). Amano H, Itakura K, Maruyama M, Ichisaka T, Nakagawa M, Yamanaka S. BMC Dev Biol. 2006 . 6():11. 10.1186/1471-213X-6-11 PubMed 16504174
Map uploaded by the depositor.