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p3xFLAG SARS-CoV2 RdRp.co
(Plasmid #154008)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 154008 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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Backbone

  • Vector backbone
    p3xFLAG-CMV-14
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6310
  • Total vector size (bp) 9159
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Codon-optimized SARS-CoV2 Nsp12 from ORF1ab
  • Alt name
    SARS-CoV2 RdRp
  • Species
    Synthetic
  • Insert Size (bp)
    2892
  • GenBank ID
    MN985325.1
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer 3xFLAG reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Insert DNA was synthesized by IDT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG SARS-CoV2 RdRp.co was a gift from Patrick Moore (Addgene plasmid # 154008 ; http://n2t.net/addgene:154008 ; RRID:Addgene_154008)