Addgene - FLAG-MED6 Plasmid Data

Plasmid 15411: FLAG-MED6

Gene/insert name:
MED6
Insert size:
633
Species:
M. musculus (mouse)
Entrez Gene:
Med6 (AU019660, AV213303, 1500012F11Rik)
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Mutation:
N/A
Vector backbone:
pcDNA3.1 N-FLAG/Hyg
(Search Vector Database)
Backbone manufacturer:
Invitrogen, modified at Conaway lab
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5600
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
BGH reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (1)
Principal Investigator:
Joan Conaway
Principal Investigator:
Ronald Conaway
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex. Sato et al (J Biol Chem. 2003 Dec 12. 278(50):49671-4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15411" in your Materials and Methods section.