pDYU1H-TED
(Plasmid
#154300)
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Purpose(Empty Backbone) A expression vector in Dictyostelium discoideum containing the Hygromycin B resistant gene and the TED module for GFP-negative screening
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 154300 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDYU1H
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Backbone manufacturerTomo Kondo
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Modifications to backboneThe TED module is inserted.
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Vector typeDictyostelium Expression
- Promoter actin15
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS result have some ambiguous bases in the backbone, depositor confirmed that these bases do not affect the plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDYU1H-TED was a gift from Shigehiko Yumura (Addgene plasmid # 154300 ; http://n2t.net/addgene:154300 ; RRID:Addgene_154300) -
For your References section:
An improved molecular tool for screening bacterial colonies using GFP expression enhanced by a Dictyostelium sequence. Kondo T, Yumura S. Biotechniques. 2020 Feb;68(2):91-95. doi: 10.2144/btn-2019-0127. Epub 2019 Dec 11. 10.2144/btn-2019-0127 PubMed 31825246
